Zooplankton Collections for EEGLE Zooplankton were collected at each station with a 50 cm, 153-um mesh net equipped with a calibrated flowmeter and hauled from approximately 2 m off the lake bottom to the surface. Two replicate samples were collected at each station. Zooplankton Abundance In the laboratory, two or more subsamples were taken with a Stempel pipette for each sample collected until at least 600 animals were identified. All adult copepods and cladocerans in each subsample were identified to sex and species. Immature copepodites were identified to genus, while nauplii were combined into one group. The mean abundance of the two replicates is presented as the number of organisms per cubic meter. Zooplankton Biomass Lengths were determined for those animals that contributed to at least 5% of the total number of animals in the sample to estimate dry biomass from length-weight regression equations from the literature. Mean lengths were averaged from 10 individuals of both adult male and female copepods and 25 individuals for nauplii, copepodites, and cladocerans. Default weights from the literature were used for rare taxa that were not measured. Mean dry biomass is presented as milligrams per cubic meter. Note Zooplankton samples were collected with a 64-um mesh net in addition to the 153-um mesh net, which does not efficiently sample small nauplii. There may be additional data in the future for nauplii abundance and biomass estimates from the smaller sized net. Mysis relicta numbers are not reliable because they must be sampled at night using a special net. Contact Information Megan Agy or Henry Vanderploeg NOAA/Great Lakes Environmental Research Laboratory 2205 Commonwealth Blvd. Ann Arbor, Michigan 48105. Phone number: (734) 741-2284 E-mail: henry.vanderploeg@noaa.gov